diff --git a/packages/@aws-cdk/aws-apigatewayv2/README.md b/packages/@aws-cdk/aws-apigatewayv2/README.md
index 70bff02cf7ea8..cc3132a9a072a 100644
--- a/packages/@aws-cdk/aws-apigatewayv2/README.md
+++ b/packages/@aws-cdk/aws-apigatewayv2/README.md
@@ -34,6 +34,7 @@ Higher level constructs for Websocket APIs | ![Experimental](https://img.shields
   - [Cross Origin Resource Sharing (CORS)](#cross-origin-resource-sharing-cors)
   - [Publishing HTTP APIs](#publishing-http-apis)
   - [Custom Domain](#custom-domain)
+  - [Mutual TLS](#mutual-tls-mtls)
   - [Managing access](#managing-access)
   - [Metrics](#metrics)
   - [VPC Link](#vpc-link)
@@ -254,6 +255,29 @@ declare const apiDemo: apigwv2.HttpApi;
 const demoDomainUrl = apiDemo.defaultStage?.domainUrl; // returns "https://example.com/demo"
 ```
 
+## Mutual TLS (mTLS)
+
+Mutual TLS can be configured to limit access to your API based by using client certificates instead of (or as an extension of) using authorization headers.
+
+```ts
+import * as s3 from '@aws-cdk/aws-s3';
+const certArn = 'arn:aws:acm:us-east-1:111111111111:certificate';
+const domainName = 'example.com';
+const bucket = new s3.Bucket.fromBucketName(stack, 'TrustStoreBucket', ...);
+
+new DomainName(stack, 'DomainName', {
+  domainName,
+  certificate: Certificate.fromCertificateArn(stack, 'cert', certArn),
+  mtls: {
+    bucket,
+    key: 'someca.pem',
+    version: 'version',
+  },
+})
+```
+
+Instructions for configuring your trust store can be found [here](https://aws.amazon.com/blogs/compute/introducing-mutual-tls-authentication-for-amazon-api-gateway/)
+
 ### Managing access
 
 API Gateway supports multiple mechanisms for [controlling and managing access to your HTTP
diff --git a/packages/@aws-cdk/aws-apigatewayv2/lib/common/domain-name.ts b/packages/@aws-cdk/aws-apigatewayv2/lib/common/domain-name.ts
index 6b1123512c678..e550a21f915f3 100644
--- a/packages/@aws-cdk/aws-apigatewayv2/lib/common/domain-name.ts
+++ b/packages/@aws-cdk/aws-apigatewayv2/lib/common/domain-name.ts
@@ -1,4 +1,5 @@
 import { ICertificate } from '@aws-cdk/aws-certificatemanager';
+import { IBucket } from '@aws-cdk/aws-s3';
 import { IResource, Resource, Token } from '@aws-cdk/core';
 import { Construct } from 'constructs';
 import { CfnDomainName, CfnDomainNameProps } from '../apigatewayv2.generated';
@@ -59,6 +60,32 @@ export interface DomainNameProps {
    * The ACM certificate for this domain name
    */
   readonly certificate: ICertificate;
+  /**
+   * The mutual TLS authentication configuration for a custom domain name.
+   * @default - mTLS is not configured.
+   */
+  readonly mtls?: MTLSConfig
+}
+
+/**
+ * The mTLS authentication configuration for a custom domain name.
+ */
+export interface MTLSConfig {
+  /**
+   * The bucket that the trust store is hosted in.
+   */
+  readonly bucket: IBucket;
+  /**
+   * The key in S3 to look at for the trust store
+   */
+  readonly key: string;
+
+  /**
+   *  The version of the S3 object that contains your truststore.
+   *  To specify a version, you must have versioning enabled for the S3 bucket.
+   *  @default - latest version
+   */
+  readonly version?: string;
 }
 
 /**
@@ -88,6 +115,7 @@ export class DomainName extends Resource implements IDomainName {
       throw new Error('empty string for domainName not allowed');
     }
 
+    const mtlsConfig = this.configureMTLS(props.mtls);
     const domainNameProps: CfnDomainNameProps = {
       domainName: props.domainName,
       domainNameConfigurations: [
@@ -96,10 +124,19 @@ export class DomainName extends Resource implements IDomainName {
           endpointType: 'REGIONAL',
         },
       ],
+      mutualTlsAuthentication: mtlsConfig,
     };
     const resource = new CfnDomainName(this, 'Resource', domainNameProps);
     this.name = resource.ref;
     this.regionalDomainName = Token.asString(resource.getAtt('RegionalDomainName'));
     this.regionalHostedZoneId = Token.asString(resource.getAtt('RegionalHostedZoneId'));
   }
+
+  private configureMTLS(mtlsConfig?: MTLSConfig): CfnDomainName.MutualTlsAuthenticationProperty | undefined {
+    if (!mtlsConfig) return undefined;
+    return {
+      truststoreUri: mtlsConfig.bucket.s3UrlForObject(mtlsConfig.key),
+      truststoreVersion: mtlsConfig.version,
+    };
+  }
 }
diff --git a/packages/@aws-cdk/aws-apigatewayv2/package.json b/packages/@aws-cdk/aws-apigatewayv2/package.json
index 4ce2dbc057014..121096724bdfd 100644
--- a/packages/@aws-cdk/aws-apigatewayv2/package.json
+++ b/packages/@aws-cdk/aws-apigatewayv2/package.json
@@ -89,6 +89,7 @@
     "@aws-cdk/aws-cloudwatch": "0.0.0",
     "@aws-cdk/aws-ec2": "0.0.0",
     "@aws-cdk/aws-iam": "0.0.0",
+    "@aws-cdk/aws-s3": "0.0.0",
     "@aws-cdk/core": "0.0.0",
     "constructs": "^3.3.69"
   },
@@ -97,6 +98,7 @@
     "@aws-cdk/aws-cloudwatch": "0.0.0",
     "@aws-cdk/aws-ec2": "0.0.0",
     "@aws-cdk/aws-iam": "0.0.0",
+    "@aws-cdk/aws-s3": "0.0.0",
     "@aws-cdk/core": "0.0.0",
     "constructs": "^3.3.69"
   },
diff --git a/packages/@aws-cdk/aws-apigatewayv2/test/http/domain-name.test.ts b/packages/@aws-cdk/aws-apigatewayv2/test/http/domain-name.test.ts
index 2d0d856c7ae15..9c60f7b5196e3 100644
--- a/packages/@aws-cdk/aws-apigatewayv2/test/http/domain-name.test.ts
+++ b/packages/@aws-cdk/aws-apigatewayv2/test/http/domain-name.test.ts
@@ -1,5 +1,6 @@
 import { Template } from '@aws-cdk/assertions';
 import { Certificate } from '@aws-cdk/aws-certificatemanager';
+import { Bucket } from '@aws-cdk/aws-s3';
 import { Stack } from '@aws-cdk/core';
 import { DomainName, HttpApi } from '../../lib';
 
@@ -168,4 +169,66 @@ describe('DomainName', () => {
     expect(t).toThrow('defaultDomainMapping not supported with createDefaultStage disabled');
 
   });
+
+  test('accepts a mutual TLS configuration', () => {
+    // GIVEN
+    const stack = new Stack();
+    const bucket = Bucket.fromBucketName(stack, 'testBucket', 'example-bucket');
+
+    // WHEN
+    new DomainName(stack, 'DomainName', {
+      domainName,
+      certificate: Certificate.fromCertificateArn(stack, 'cert', certArn),
+      mtls: {
+        bucket,
+        key: 'someca.pem',
+      },
+    });
+
+    // THEN
+    Template.fromStack(stack).hasResourceProperties('AWS::ApiGatewayV2::DomainName', {
+      DomainName: 'example.com',
+      DomainNameConfigurations: [
+        {
+          CertificateArn: 'arn:aws:acm:us-east-1:111111111111:certificate',
+          EndpointType: 'REGIONAL',
+        },
+      ],
+      MutualTlsAuthentication: {
+        TruststoreUri: 's3://example-bucket/someca.pem',
+      },
+    });
+  });
+
+  test('mTLS should allow versions to be set on the s3 bucket', () => {
+    // GIVEN
+    const stack = new Stack();
+    const bucket = Bucket.fromBucketName(stack, 'testBucket', 'example-bucket');
+
+    // WHEN
+    new DomainName(stack, 'DomainName', {
+      domainName,
+      certificate: Certificate.fromCertificateArn(stack, 'cert', certArn),
+      mtls: {
+        bucket,
+        key: 'someca.pem',
+        version: 'version',
+      },
+    });
+
+    // THEN
+    Template.fromStack(stack).hasResourceProperties('AWS::ApiGatewayV2::DomainName', {
+      DomainName: 'example.com',
+      DomainNameConfigurations: [
+        {
+          CertificateArn: 'arn:aws:acm:us-east-1:111111111111:certificate',
+          EndpointType: 'REGIONAL',
+        },
+      ],
+      MutualTlsAuthentication: {
+        TruststoreUri: 's3://example-bucket/someca.pem',
+        TruststoreVersion: 'version',
+      },
+    });
+  });
 });