From d8ebafe2e5ae6dc5dbdc284fb81b9b479418bfee Mon Sep 17 00:00:00 2001 From: Andrey Ershov Date: Sun, 24 Sep 2023 08:24:15 +0200 Subject: [PATCH] Resolves #3 adding gene expr correlation with str length in crc support + docker-compose + alembic migrations (optional) --- .devcontainer/devcontainer.json | 29 + .dockerignore | 28 + .env.example | 7 + .gitignore | 5 + .vscode/launch.json | 46 + .vscode/tasks.json | 43 + Dockerfile | 25 +- README.md | 50 +- alembic.ini | 110 + data/genexp_str_length_correlations.csv | 15380 ++++++++++++++++ database_setup/add_genomes.py | 6 +- database_setup/add_panels_and_cohorts.py | 32 +- database_setup/full_db_setup.sh | 11 +- .../import_crc_expr_repeatlength_corr.py | 125 + database_setup/migrations/__init__.py | 0 database_setup/migrations/env.py | 91 + database_setup/migrations/script.py.mako | 23 + .../versions/2023_08_26_1750-31a133f5e181_.py | 297 + .../versions/2023_08_27_1759-edc492faa8d0_.py | 31 + .../versions/2023_09_03_1728-09734b51018c_.py | 53 + docker-compose.yml | 55 + entrypoint.sh | 26 + requirements.txt | 11 +- strAPI/main.py | 28 +- strAPI/repeats/database.py | 53 +- strAPI/repeats/models.py | 43 +- strAPI/repeats/schemas.py | 21 +- 27 files changed, 16585 insertions(+), 44 deletions(-) create mode 100644 .devcontainer/devcontainer.json create mode 100644 .dockerignore create mode 100644 .env.example create mode 100644 .vscode/launch.json create mode 100644 .vscode/tasks.json create mode 100644 alembic.ini create mode 100644 data/genexp_str_length_correlations.csv create mode 100644 database_setup/import_crc_expr_repeatlength_corr.py create mode 100644 database_setup/migrations/__init__.py create mode 100644 database_setup/migrations/env.py create mode 100644 database_setup/migrations/script.py.mako create mode 100644 database_setup/migrations/versions/2023_08_26_1750-31a133f5e181_.py create mode 100644 database_setup/migrations/versions/2023_08_27_1759-edc492faa8d0_.py create mode 100644 database_setup/migrations/versions/2023_09_03_1728-09734b51018c_.py create mode 100644 docker-compose.yml create mode 100644 entrypoint.sh diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json new file mode 100644 index 0000000..c3be748 --- /dev/null +++ b/.devcontainer/devcontainer.json @@ -0,0 +1,29 @@ +// For format details, see https://aka.ms/devcontainer.json. For config options, see the +// README at: https://github.com/devcontainers/templates/tree/main/src/docker-existing-dockerfile +{ + "name": "Existing Dockerfile", + "build": { + // Sets the run context to one level up instead of the .devcontainer folder. + "context": "..", + // Update the 'dockerFile' property if you aren't using the standard 'Dockerfile' filename. + "dockerfile": "../Dockerfile" + }, + "features": { + "ghcr.io/devcontainers/features/python:1": {} + } + + // Features to add to the dev container. More info: https://containers.dev/features. + // "features": {}, + + // Use 'forwardPorts' to make a list of ports inside the container available locally. + // "forwardPorts": [], + + // Uncomment the next line to run commands after the container is created. + // "postCreateCommand": "cat /etc/os-release", + + // Configure tool-specific properties. + // "customizations": {}, + + // Uncomment to connect as an existing user other than the container default. More info: https://aka.ms/dev-containers-non-root. + // "remoteUser": "devcontainer" +} diff --git a/.dockerignore b/.dockerignore new file mode 100644 index 0000000..c91e23d --- /dev/null +++ b/.dockerignore @@ -0,0 +1,28 @@ +**/__pycache__ +**/.venv +**/.classpath +**/.dockerignore +**/.env +**/.git +**/.gitignore +**/.project +**/.settings +**/.toolstarget +**/.vs +**/.vscode +**/*.*proj.user +**/*.dbmdl +**/*.jfm +**/bin +**/charts +**/docker-compose* +**/compose* +**/Dockerfile* +**/node_modules +**/npm-debug.log +**/obj +**/secrets.dev.yaml +**/values.dev.yaml +**/db/ +LICENSE +README.md diff --git a/.env.example b/.env.example new file mode 100644 index 0000000..4d1f6a3 --- /dev/null +++ b/.env.example @@ -0,0 +1,7 @@ +# Rename this file to .env and docker compose wil automatically use envornment variables fro it +DATABASE_URL=postgresql://webstr:webstr@db:5432/webstr +# DATABASE_URL=sqlite:///db/debug.sqlite +PYTHONPATH="./" +WEBSTR_DATABASE_DATA_UPGRADE=True +WEBSTR_DEVELOPMENT=False +WEBSTR_SOURCE_MOUNT_PATH=/temp/src #/usr/src/strs \ No newline at end of file diff --git a/.gitignore b/.gitignore index 675d000..faf8541 100644 --- a/.gitignore +++ b/.gitignore @@ -4,4 +4,9 @@ strAPI/local_queries.ipynb data/repeats +db/docker_data/ .venv/ +db/debug.db + +*/migrations/versions0/* +*.env diff --git a/.vscode/launch.json b/.vscode/launch.json new file mode 100644 index 0000000..b0ac4d7 --- /dev/null +++ b/.vscode/launch.json @@ -0,0 +1,46 @@ +{ + "configurations": [ + { + "name": "Python: main", + "type": "python", + "request": "launch", + "program": "strAPI/main.py", + "cwd": "${workspaceFolder}", + "console": "integratedTerminal", + "justMyCode": true, + "env": { + // "PYTHONPATH": "${workspaceFolder}/strAPI/" + } + }, + { + "name": "Docker: Python - Fastapi", + "type": "docker", + "request": "launch", + "preLaunchTask": "docker-run: debug", + "python": { + "pathMappings": [ + { + "localRoot": "${workspaceFolder}", + "remoteRoot": "/usr/src/strs" + } + ], + "projectType": "fastapi" + }, + }, + { + "name": "Python: Remote Attach 5000", + "type": "python", + "request": "attach", + "connect": { + "host": "0.0.0.0", + "port": 5000 + }, + "pathMappings": [ + { + "localRoot": "${workspaceFolder}", + "remoteRoot": "/usr/src/app" + } + ] + } + ] +} \ No newline at end of file diff --git a/.vscode/tasks.json b/.vscode/tasks.json new file mode 100644 index 0000000..09022fa --- /dev/null +++ b/.vscode/tasks.json @@ -0,0 +1,43 @@ +{ + "version": "2.0.0", + "tasks": [ + { + "type": "docker-build", + "label": "docker-build", + "platform": "python", + "dockerBuild": { + "tag": "webstrapi:latest", + "dockerfile": "${workspaceFolder}/Dockerfile", + "context": "${workspaceFolder}", + "pull": true, + } + }, + { + "type": "docker-run", + "label": "docker-run: debug", + "dependsOn": [ + "docker-build" + ], + "dockerRun": { + "env": { + "DATABASE_URL": "sqlite:///db/debug.db", + "WEBSTR_DATABAS_UPGRADE": "True" + }, + "mounts": [ + "source=${workspaceFolder},target=/app,type=bind,consistency=cached" + ], + }, + "python": { + "args": [ + "strAPI.main:app", + "--host", + "0.0.0.0", + "--port", + "5000", + "--reload" + ], + "module": "uvicorn", + } + } + ] +} \ No newline at end of file diff --git a/Dockerfile b/Dockerfile index ef1bf30..f1bcbc5 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,18 +1,33 @@ -FROM python:3.8-slim-buster +FROM python:3.8-slim-buster as base LABEL maintainer="merenlin -- follow me on medium https://medium.com/@merenlin" -RUN apt-get update && apt-get install -y python3-dev build-essential +ENV PYTHONDONTWRITEBYTECODE=1 +ENV PYTHONUNBUFFERED=1 RUN mkdir -p /usr/src/strs WORKDIR /usr/src/strs COPY requirements.txt . -RUN pip3 install -r requirements.txt + +RUN apt-get update && \ + apt-get install -y gcc python3-dev build-essential && \ + python -m pip install -r requirements.txt --no-cache-dir && \ + apt-get remove -y gcc python3-dev build-essential && \ + apt-get clean && rm -rf /var/lib/apt/lists/* + +ENV DATABASE_URL=sqlite:///db/debug.db + +FROM base as prod +LABEL maintainer="merenlin -- follow me on medium https://medium.com/@merenlin" + +RUN adduser -u 1000 --disabled-password --gecos "" appuser && chown -R appuser /usr/src/strs +USER appuser COPY . . -EXPOSE 5000 +ARG PORT=5000 +EXPOSE $PORT -CMD uvicorn strAPI.main:app --host=0.0.0.0 --port=${PORT:-5000} +CMD bash entrypoint.sh diff --git a/README.md b/README.md index c1050b4..90b9e7f 100644 --- a/README.md +++ b/README.md @@ -23,10 +23,17 @@ Yes! It is possible and we encourage it if you would like to add your own data t ### Instructions on how to set-up webSTR-API locally (for development): -#### Step 0: Set up the database +#### Step 0: (docker and local ways) Set up the database Install and configure PostgreSQL on your machine and create an empty database called strdb. We provide an sql_dump backup of the current version of the database on request. Restore the database from this backup. - -#### Step 1: Install all the requirements + + Alternatively you can use `docker compose` with provided docker-compose.yml file to set up the PostgreSQL database. + 1. rename `.env.example` to `.env` + 2. copy backup to folder ./db/docker_data/pgdata/webstr_backup.dump + 2. `docker compose up -d db` + 3. `docker exec -it webstr-api-db-1 pg_restore -d strdb /var/lib/postgresql/data/pgdata/webstr_backup.dump` + +#### Step 1: (only for non-docker way) Install all the requirements + a) Set up python3 and virtualenv on your machine: [For Mac, follow instructions here.](https://gist.github.com/pandafulmanda/730a9355e088a9970b18275cb9eadef3) @@ -38,22 +45,40 @@ b) Create new virtual env and install all the requirements with the following co `pip install -r requirements.txt` -#### Step 2: Set environmental variable DATABASE_URL on your machine (or your IDE) to - +#### Step 2: (only for non-docker way) Set environmental variable DATABASE_URL on your machine (or your IDE) to `export DATABASE_URL="postgres://postgres:YOURPASSWORD@localhost:5432/strdb"` Note that this is using the default user postgres, if you created your db on a different user, adjust this variable accordingly. Optional: add this line to `~/.bashrc` and restart your terminal. -#### Step 3: Start API server +#### Step 3: (only for non-docker way) Start API server Run the following command from the root folder of this repo: `uvicorn strAPI.main:app --host=0.0.0.0 --port=${PORT:-5000} --reload` -#### Step 4: You can now access the api at `http://0.0.0.0:5000` +#### Step 4: You can now access the api at `http://localhost:5000` + +*** +### How to build and run application using docker way + +#### Step 0: Set up the database +##### if you want to use PostgreSQL and backup +See docker part of Step 1 from the previous instructions +##### if sqlite is enough +Change database url in .env file to DATABASE_URL=sqlite:///db/debug.sqlite +Set WEBSTR_DATABASE_DATA_UPGRADE=True and WEBSTR_DEVELOPMENT=True in .env file +#### Step 1: Start containers +Run `docker compose up` + +Now you can access api on localhost:5000 and frontend on localhost:5001 + +##### debugging in docker container +If you want to debug the code and you use VSCode - you can run code in container using vscode and tasks defined in launch.json in .vscode folder. Or you can use devcontaienr plugin to do the same. This is a bit more advanced, so you need to study how debuggin in containers works in vscode a bit before that. And you will have to stop webster-api-api-1 container first if you have it running through docker compose. + +*** ## How to build the database from scratch or import my own data to WebSTR? @@ -76,3 +101,14 @@ Explore "database_setup" directory for different utilities to import data into t * If you would like **to import a new reference panel** we recommend making a csv corresponding to the repeats table structure and importing it directly to SQL to save time. Alternatively see ` insert_repeats.py ` and ` import_data_ensembltrs.py ` utilities that we made for repeats data coming in different formats. Feel free to contact us for more details if you would like to make your own reference STR panel. +*** + +### Database migrations using Alembic - Proof of Concept, not used in production. +@slmjy added alembic migrations as a proof of concept to the codebase. +Following changes were introduced: +1. Database migrations in /database_setup/migrations +2. alembic.ini and env.py files +3. entrypoint.sh can run alembic migratiosn given environment variable WEBSTR_DATABASE_MIGRATE is set to True +4. in database.py there is a disabled check if database is on the latest version + +If someone wants to start using alembic migrations, they can enable the version check and start generating new migrations using alembic and using them in production. \ No newline at end of file diff --git a/alembic.ini b/alembic.ini new file mode 100644 index 0000000..587fec0 --- /dev/null +++ b/alembic.ini @@ -0,0 +1,110 @@ +# A generic, single database configuration. + +[alembic] +# path to migration scripts +script_location = database_setup/migrations + +# template used to generate migration file names; The default value is %%(rev)s_%%(slug)s +# Uncomment the line below if you want the files to be prepended with date and time +# see https://alembic.sqlalchemy.org/en/latest/tutorial.html#editing-the-ini-file +# for all available tokens +file_template = %%(year)d_%%(month).2d_%%(day).2d_%%(hour).2d%%(minute).2d-%%(rev)s_%%(slug)s + +# sys.path path, will be prepended to sys.path if present. +# defaults to the current working directory. +prepend_sys_path = . + +# timezone to use when rendering the date within the migration file +# as well as the filename. +# If specified, requires the python-dateutil library that can be +# installed by adding `alembic[tz]` to the pip requirements +# string value is passed to dateutil.tz.gettz() +# leave blank for localtime +# timezone = + +# max length of characters to apply to the +# "slug" field +# truncate_slug_length = 40 + +# set to 'true' to run the environment during +# the 'revision' command, regardless of autogenerate +# revision_environment = false + +# set to 'true' to allow .pyc and .pyo files without +# a source .py file to be detected as revisions in the +# versions/ directory +# sourceless = false + +# version location specification; This defaults +# to migrations/versions. When using multiple version +# directories, initial revisions must be specified with --version-path. +# The path separator used here should be the separator specified by "version_path_separator" below. +# version_locations = %(here)s/bar:%(here)s/bat:migrations/versions + +# version path separator; As mentioned above, this is the character used to split +# version_locations. The default within new alembic.ini files is "os", which uses os.pathsep. +# If this key is omitted entirely, it falls back to the legacy behavior of splitting on spaces and/or commas. +# Valid values for version_path_separator are: +# +# version_path_separator = : +# version_path_separator = ; +# version_path_separator = space +version_path_separator = os # Use os.pathsep. Default configuration used for new projects. + +# set to 'true' to search source files recursively +# in each "version_locations" directory +# new in Alembic version 1.10 +# recursive_version_locations = false + +# the output encoding used when revision files +# are written from script.py.mako +# output_encoding = utf-8 + +sqlalchemy.url = sqlite:///db/debug.sqlite + + +[post_write_hooks] +# post_write_hooks defines scripts or Python functions that are run +# on newly generated revision scripts. See the documentation for further +# detail and examples + +# format using "black" - use the console_scripts runner, against the "black" entrypoint +# hooks = black +# black.type = console_scripts +# black.entrypoint = black +# black.options = -l 79 REVISION_SCRIPT_FILENAME + +# Logging configuration +[loggers] +keys = root,sqlalchemy,alembic + +[handlers] +keys = console + +[formatters] +keys = generic + +[logger_root] +level = WARN +handlers = console +qualname = + +[logger_sqlalchemy] +level = WARN +handlers = +qualname = sqlalchemy.engine + +[logger_alembic] +level = INFO +handlers = +qualname = alembic + +[handler_console] +class = StreamHandler +args = (sys.stderr,) +level = NOTSET +formatter = generic + +[formatter_generic] +format = %(levelname)-5.5s [%(name)s] %(message)s +datefmt = %H:%M:%S diff --git a/data/genexp_str_length_correlations.csv b/data/genexp_str_length_correlations.csv new file mode 100644 index 0000000..3dcfed4 --- /dev/null +++ b/data/genexp_str_length_correlations.csv @@ -0,0 +1,15380 @@ +gene,tmp_id,coefficient,pvalue_coef,intercept,pvalue_corrected +ENSG00000121716,chr7_100359506,-1.0991624329058038,5.756609826569003e-25,4.046082934307631,8.853090252280471e-21 +ENSG00000102796,chr13_51791272,0.470840239330189,5.39514323615449e-16,-5.924928473594489,4.148595391440995e-12 +ENSG00000101473,chr20_45842010,0.6060933839759207,5.033235247867376e-15,-7.058699021605443,2.580204162565079e-11 +ENSG00000112414,chr6_142370814,-0.432043423155773,7.364989684102237e-15,6.573100680523672,2.8316544087952076e-11 +ENSG00000241962,chr2_99319803,-0.3699702597231034,1.1949832013567806e-13,4.748427205682092,3.675529330733186e-10 +ENSG00000151247,chr4_98881150,-0.5849125719222736,2.884563376180399e-13,5.995575374701825,6.895793484779627e-10 +ENSG00000116514,chr1_32936734,-0.3314766429612769,3.138731672635242e-13,5.209345681290323,6.895793484779627e-10 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+ENSG00000127324,chr12_71129420,7.073722755161597e-05,0.9992975407425488,0.0091736694861786,0.9996875612489208 +ENSG00000197121,chr2_196880156,4.735203884585586e-05,0.9996319600971736,0.0103555772667797,0.999920950730698 +ENSG00000165997,chr10_18668771,3.8050159951333445e-05,0.9996608763563614,-0.4411812712220572,0.999920950730698 +ENSG00000231925,chr6_33315144,-5.49069988307943e-05,0.9998027538668592,-0.0987231218399214,0.9999978246434982 +ENSG00000162999,chr2_183083414,1.0209407472622225e-06,0.9999954270282144,-0.0032688949238162,0.9999999999999358 +ENSG00000125952,chr14_65076348,-1.198042468616295e-07,0.9999995267226808,0.135157825062562,0.9999999999999358 +ENSG00000111218,chr12_3593077,5.698625120266597e-14,0.9999999999999358,-0.0631193214681184,0.9999999999999358 \ No newline at end of file diff --git a/database_setup/add_genomes.py b/database_setup/add_genomes.py index 828a277..28984cf 100644 --- a/database_setup/add_genomes.py +++ b/database_setup/add_genomes.py @@ -37,8 +37,10 @@ def main(): {'name': 'rn7', 'version': '', 'organism': 'Rattus norvegicus'}] for g in list_of_genomes: - g_obj = make_db_genome(g) - session.add(g_obj) + existing_genome = session.query(Genome).filter(Genome.name == g["name"]).first() + if not existing_genome: + g_obj = make_db_genome(g) + session.add(g_obj) session.commit() if __name__ == "__main__": diff --git a/database_setup/add_panels_and_cohorts.py b/database_setup/add_panels_and_cohorts.py index e60041d..c822f7e 100644 --- a/database_setup/add_panels_and_cohorts.py +++ b/database_setup/add_panels_and_cohorts.py @@ -8,14 +8,19 @@ def make_db_trpanel(session, trpanel_info): - # initialize instance of TR Panel object - db_panel = TRPanel( - name = trpanel_info['name'], - method = trpanel_info['method'] - ) + db_panel = session.query(TRPanel).filter( + TRPanel.name == trpanel_info['name'] and TRPanel.method == trpanel_info['method'] + ).first() + + if not db_panel: + # initialize instance of TR Panel object + db_panel = TRPanel( + name = trpanel_info['name'], + method = trpanel_info['method'] + ) # get corresponding genome and assign to panel - genome = session.query(Genome).filter(Genome.name == trpanel_info['genome']).one() + genome = session.query(Genome).filter(Genome.name == trpanel_info['genome']).first() genome.trpanels.append(db_panel) db_panel.genome_id = genome.id @@ -23,13 +28,18 @@ def make_db_trpanel(session, trpanel_info): def make_db_cohort(session, cohort_info): - # initialize instance of TR Panel object - db_cohort = Cohort( - name = cohort_info['name'], - ) + db_cohort = session.query(Cohort).filter( + Cohort.name == cohort_info['name'] + ).first() + + if not db_cohort: + # initialize instance of TR Panel object + db_cohort = Cohort( + name = cohort_info['name'], + ) # get corresponding genome and assign to panel - trpanel = session.query(TRPanel).filter(TRPanel.name == cohort_info['panel']).one() + trpanel = session.query(TRPanel).filter(TRPanel.name == cohort_info['panel']).first() #print(trpanel) trpanel.cohorts.append(db_cohort) db_cohort.trpanel_id = trpanel.id diff --git a/database_setup/full_db_setup.sh b/database_setup/full_db_setup.sh index 0f3a8f9..8674c58 100644 --- a/database_setup/full_db_setup.sh +++ b/database_setup/full_db_setup.sh @@ -1,15 +1,13 @@ #!/bin/bash set -eou pipefail - gtf="../data/genome_anntotation/gencode.v22.annotation.gtf" repeat_dir="../data/repeats" #change password to yours before running -db=postgresql+psycopg2://postgres:YOURPASSWORD@localhost:5432/strdb - +db=${DATABASE_URL:-postgresql+psycopg2://postgres:YOURPASSWORD@localhost:5432/strdb} -echo "Setting up database file" +echo "Setting up database file" python setup_db.py --database "${db}" #echo "Adding genomes" @@ -25,4 +23,7 @@ python setup_db.py --database "${db}" #python insert_repeats.py -d "${db}" -r "${repeat_dir}" -s phylo_gap01 #echo "Inserting locus level variation" -#python update_repeats.py -d "${db}" -v ../data/20220527_locus_variation_no_groups.csv +#python insert_variations.py -d "${db}" -v ../data/20220527_locus_variation_no_groups.csv + +#echo "Inserting locus level variation" +python import_crc_expr_repeatlength_corr.py -d "${db}" -f ../data/genexp_str_length_correlations.csv \ No newline at end of file diff --git a/database_setup/import_crc_expr_repeatlength_corr.py b/database_setup/import_crc_expr_repeatlength_corr.py new file mode 100644 index 0000000..b647d4a --- /dev/null +++ b/database_setup/import_crc_expr_repeatlength_corr.py @@ -0,0 +1,125 @@ +""" +This script imports gene expression and repeat length correlation data from a csv file into a database. +It uses the gtf_to_sql module to connect to the database and the pandas module to read the csv file. +It inserts data into CRCExprRepeatLenCorr model. + +The script defines a command-line interface using the argparse module. +The script can be run from the command line with the --database and --file arguments to specify the database and csv file paths. + +Script can be run multiple times. It will update existing records and insert new ones. +""" + +#!/usr/bin/env python3 +import argparse +import logging +from gtf_to_sql import connection_setup +import pandas as pd + +from strAPI.repeats.models import Gene, Repeat, CRCExprRepeatLenCorr + + +def update_db_record(df_row, session, genes_by_code, inserted_entities): + + def get_repeat(tmp_id, range=False): + chr, start = tmp_id.split("_") + start = int(start) + query = session.query(Repeat).filter_by(chr=chr.lower()) + if range: + query = query.filter(Repeat.start<=start).filter(Repeat.end>start) + else: + query = query.filter_by(start=start) + return query.first() + + repeat = get_repeat(df_row["tmp_id"].strip()) + gene = genes_by_code.get(df_row["gene"].strip()) + + repeat_id = None + gene_id = None + + if repeat is None: + logging.info( + f"Repeat {len(inserted_entities)} with code {df_row['tmp_id']} not found in database. Trying range search.") + repeat = get_repeat(df_row["tmp_id"].strip(), range=True) + if repeat is None: + logging.info( + f"Repeat {len(inserted_entities)} with code {df_row['tmp_id']} not found in database.") + + if gene is None: + logging.info( + f"Gene {len(inserted_entities)} with code {df_row['gene']} not found in database.") + + if repeat is not None and gene is not None: + gene_id = gene.id + repeat_id = repeat.id + else: + return + + entity = session.query(CRCExprRepeatLenCorr).filter_by( + repeat_id=repeat_id, + gene_id=gene_id + ).first() + + if entity is None: + + entity = CRCExprRepeatLenCorr( + repeat_id = repeat_id, + gene_id = gene_id + ) + + session.add(entity) + + entity.gene_id = gene_id + entity.repeat_id = repeat_id + entity.coefficient = df_row["coefficient"] + entity.intercept = df_row["intercept"] + entity.p_value = df_row["pvalue_coef"] + entity.p_value_corrected = df_row["pvalue_corrected"] + + inserted_entities.append(entity) + + +def cla_parser(): + parser = argparse.ArgumentParser() + + parser.add_argument( + "--database", "-d", type=str, required=True, help="Path to where the repeat-containing database can be found" + ) + parser.add_argument( + "--file", "-f", type=str, required=True, help="Path to variations file in csv format" + ) + + return parser.parse_args() + + +def main(): + args = cla_parser() + db_path = args.database + input_path = args.file + logging.info("Connecting to the database") + engine, session = connection_setup(db_path) + + inserted_entities = [] + + logging.info("Getting all genes from database") + genes_by_ensemble_code = {} + for gene in session.query(Gene).all(): + genes_by_ensemble_code[gene.ensembl_id] = gene + logging.info(f"Got {len(genes_by_ensemble_code)} genes from database") + + logging.info("Inserting gene expretion and repeat length correlation") + data_frame = pd.read_csv(input_path) + input_csv_len = len(data_frame) + data_frame.apply(lambda row: update_db_record(row, session, + genes_by_ensemble_code, inserted_entities), axis=1) + logging.info( + f""" + Inserted or updated {len(inserted_entities)} entities out of {input_csv_len} + """) + + session.commit() + + logging.info("Inserting gene expretion and repeat length correlation") + + +if __name__ == "__main__": + main() diff --git a/database_setup/migrations/__init__.py b/database_setup/migrations/__init__.py new file mode 100644 index 0000000..e69de29 diff --git a/database_setup/migrations/env.py b/database_setup/migrations/env.py new file mode 100644 index 0000000..4db5b0a --- /dev/null +++ b/database_setup/migrations/env.py @@ -0,0 +1,91 @@ +import os +import sys +from logging.config import fileConfig + +from sqlalchemy import engine_from_config +from sqlalchemy import pool +from sqlmodel import SQLModel + +from alembic import context + +# this is the Alembic Config object, which provides +# access to the values within the .ini file in use. +config = context.config + +# Interpret the config file for Python logging. +# This line sets up loggers basically. +if config.config_file_name is not None: + fileConfig(config.config_file_name) + +# Override database url with environment variable if present +config.set_main_option("sqlalchemy.url", os.environ.get("DATABASE_URL", config.get_main_option("sqlalchemy.url"))) + +# add your model's MetaData object here +# for 'autogenerate' support +# should only be used with autogenerate, otherwise it fails on non-updated database +if '--autogenerate' in sys.argv: + from strAPI.repeats.models import * + +# target_metadata = ModelBase.metadata +target_metadata = SQLModel.metadata + +# other values from the config, defined by the needs of env.py, +# can be acquired: +# my_important_option = config.get_main_option("my_important_option") +# ... etc. + + +def run_migrations_offline() -> None: + """Run migrations in 'offline' mode. + + This configures the context with just a URL + and not an Engine, though an Engine is acceptable + here as well. By skipping the Engine creation + we don't even need a DBAPI to be available. + + Calls to context.execute() here emit the given string to the + script output. + + """ + + context.configure( + target_metadata=target_metadata, + literal_binds=True, + dialect_opts={"paramstyle": "named"}, + dialect_name="postgresql", + render_as_batch=True + ) + + with context.begin_transaction(): + context.run_migrations() + + +def run_migrations_online() -> None: + """Run migrations in 'online' mode. + + In this scenario we need to create an Engine + and associate a connection with the context. + + """ + + connectable = engine_from_config( + config.get_section(config.config_ini_section, {}), + prefix="sqlalchemy.", + poolclass=pool.NullPool, + ) + + with connectable.connect() as connection: + context.configure( + connection=connection, + target_metadata=target_metadata, + render_as_batch=True + ) + + with context.begin_transaction(): + context.run_migrations() + + +if context.is_offline_mode(): + run_migrations_offline() +else: + run_migrations_online() diff --git a/database_setup/migrations/script.py.mako b/database_setup/migrations/script.py.mako new file mode 100644 index 0000000..d3b2d14 --- /dev/null +++ b/database_setup/migrations/script.py.mako @@ -0,0 +1,23 @@ +"""${message} + +Revision ID: ${up_revision} +Revises: ${down_revision} +Create Date: ${create_date} + +""" + +# revision identifiers, used by Alembic. +revision = ${repr(up_revision)} +down_revision = ${repr(down_revision)} + +from alembic import op +import sqlalchemy as sa +import sqlmodel +${imports if imports else ""} + +def upgrade(): + ${upgrades if upgrades else "pass"} + + +def downgrade(): + ${downgrades if downgrades else "pass"} \ No newline at end of file diff --git a/database_setup/migrations/versions/2023_08_26_1750-31a133f5e181_.py b/database_setup/migrations/versions/2023_08_26_1750-31a133f5e181_.py new file mode 100644 index 0000000..cc7e7ad --- /dev/null +++ b/database_setup/migrations/versions/2023_08_26_1750-31a133f5e181_.py @@ -0,0 +1,297 @@ +"""empty message + +Revision ID: 31a133f5e181 +Revises: None +Create Date: 2023-08-27 17:50:31.548989 + +""" + +# revision identifiers, used by Alembic. +revision = '31a133f5e181' +down_revision = None + +from alembic import op +import sqlalchemy as sa +import sqlmodel + + +def upgrade(): + # ### commands auto generated by Alembic - please adjust! ### + op.create_table('exons', + sa.Column('id', sa.Integer(), nullable=True), + sa.Column('ensembl_exon', sqlmodel.sql.sqltypes.AutoString(), nullable=False), + sa.Column('start', sa.Integer(), nullable=False), + sa.Column('end', sa.Integer(), nullable=False), + sa.Column('cds', sa.Boolean(), nullable=False), + sa.Column('start_codon', sa.Integer(), nullable=True), + sa.Column('stop_codon', sa.Integer(), nullable=True), + sa.PrimaryKeyConstraint('id'), + sa.UniqueConstraint('ensembl_exon') + ) + op.create_index(op.f('ix_exons_cds'), 'exons', ['cds'], unique=False) + op.create_index(op.f('ix_exons_end'), 'exons', ['end'], unique=False) + op.create_index(op.f('ix_exons_ensembl_exon'), 'exons', ['ensembl_exon'], unique=False) + op.create_index(op.f('ix_exons_id'), 'exons', ['id'], unique=False) + op.create_index(op.f('ix_exons_start'), 'exons', ['start'], unique=False) + op.create_index(op.f('ix_exons_start_codon'), 'exons', ['start_codon'], unique=False) + op.create_index(op.f('ix_exons_stop_codon'), 'exons', ['stop_codon'], unique=False) + op.create_table('genomes', + sa.Column('id', sa.Integer(), nullable=True), + sa.Column('name', sqlmodel.sql.sqltypes.AutoString(), nullable=False), + sa.Column('organism', sqlmodel.sql.sqltypes.AutoString(), nullable=False), + sa.Column('version', sqlmodel.sql.sqltypes.AutoString(), nullable=True), + sa.PrimaryKeyConstraint('id') + ) + op.create_index(op.f('ix_genomes_id'), 'genomes', ['id'], unique=False) + op.create_index(op.f('ix_genomes_name'), 'genomes', ['name'], unique=False) + op.create_index(op.f('ix_genomes_organism'), 'genomes', ['organism'], unique=False) + op.create_index(op.f('ix_genomes_version'), 'genomes', ['version'], unique=False) + op.create_table('genes', + sa.Column('id', sa.Integer(), nullable=True), + sa.Column('ensembl_id', sqlmodel.sql.sqltypes.AutoString(), nullable=False), + sa.Column('ensembl_version_id', sqlmodel.sql.sqltypes.AutoString(), nullable=False), + sa.Column('entrez_id', sqlmodel.sql.sqltypes.AutoString(), nullable=True), + sa.Column('name', sqlmodel.sql.sqltypes.AutoString(), nullable=True), + sa.Column('description', sqlmodel.sql.sqltypes.AutoString(), nullable=True), + sa.Column('chr', sqlmodel.sql.sqltypes.AutoString(), nullable=False), + sa.Column('strand', sqlmodel.sql.sqltypes.AutoString(), nullable=False), + sa.Column('start', sa.Integer(), nullable=False), + sa.Column('end', sa.Integer(), nullable=False), + sa.Column('genome_id', sa.Integer(), nullable=False), + sa.CheckConstraint("strand in ('+', '-')"), + sa.ForeignKeyConstraint(['genome_id'], ['genomes.id'], ), + sa.PrimaryKeyConstraint('id'), + sa.UniqueConstraint('ensembl_version_id') + ) + op.create_index(op.f('ix_genes_chr'), 'genes', ['chr'], unique=False) + op.create_index(op.f('ix_genes_description'), 'genes', ['description'], unique=False) + op.create_index(op.f('ix_genes_end'), 'genes', ['end'], unique=False) + op.create_index(op.f('ix_genes_ensembl_id'), 'genes', ['ensembl_id'], unique=False) + op.create_index(op.f('ix_genes_ensembl_version_id'), 'genes', ['ensembl_version_id'], unique=False) + op.create_index(op.f('ix_genes_entrez_id'), 'genes', ['entrez_id'], unique=False) + op.create_index(op.f('ix_genes_genome_id'), 'genes', ['genome_id'], unique=False) + op.create_index(op.f('ix_genes_id'), 'genes', ['id'], unique=False) + op.create_index(op.f('ix_genes_name'), 'genes', ['name'], unique=False) + op.create_index(op.f('ix_genes_start'), 'genes', ['start'], unique=False) + op.create_index(op.f('ix_genes_strand'), 'genes', ['strand'], unique=False) + op.create_table('trpanels', + sa.Column('id', sa.Integer(), nullable=True), + sa.Column('name', sqlmodel.sql.sqltypes.AutoString(), nullable=False), + sa.Column('method', sqlmodel.sql.sqltypes.AutoString(), nullable=False), + sa.Column('genome_id', sa.Integer(), nullable=False), + sa.ForeignKeyConstraint(['genome_id'], ['genomes.id'], ), + sa.PrimaryKeyConstraint('id') + ) + op.create_index(op.f('ix_trpanels_genome_id'), 'trpanels', ['genome_id'], unique=False) + op.create_index(op.f('ix_trpanels_id'), 'trpanels', ['id'], unique=False) + op.create_index(op.f('ix_trpanels_method'), 'trpanels', ['method'], unique=False) + op.create_index(op.f('ix_trpanels_name'), 'trpanels', ['name'], unique=False) + op.create_table('cohorts', + sa.Column('id', sa.Integer(), nullable=True), + sa.Column('name', sqlmodel.sql.sqltypes.AutoString(), nullable=False), + sa.Column('trpanel_id', sa.Integer(), nullable=False), + sa.ForeignKeyConstraint(['trpanel_id'], ['trpanels.id'], ), + sa.PrimaryKeyConstraint('id') + ) + op.create_index(op.f('ix_cohorts_id'), 'cohorts', ['id'], unique=False) + op.create_index(op.f('ix_cohorts_name'), 'cohorts', ['name'], unique=False) + op.create_index(op.f('ix_cohorts_trpanel_id'), 'cohorts', ['trpanel_id'], unique=False) + op.create_table('repeats', + sa.Column('id', sa.Integer(), nullable=False), + sa.Column('source', sqlmodel.sql.sqltypes.AutoString(), nullable=True), + sa.Column('chr', sqlmodel.sql.sqltypes.AutoString(), nullable=False), + sa.Column('msa', sqlmodel.sql.sqltypes.AutoString(), nullable=True), + sa.Column('motif', sqlmodel.sql.sqltypes.AutoString(), nullable=True), + sa.Column('start', sa.Integer(), nullable=False), + sa.Column('end', sa.Integer(), nullable=False), + sa.Column('l_effective', sa.Integer(), nullable=False), + sa.Column('n_effective', sa.Integer(), nullable=False), + sa.Column('region_length', sa.Integer(), nullable=False), + sa.Column('score_type', sqlmodel.sql.sqltypes.AutoString(), nullable=False), + sa.Column('score', sa.Float(), nullable=False), + sa.Column('p_value', sa.Float(), nullable=False), + sa.Column('divergence', sa.Float(), nullable=False), + sa.Column('trpanel_id', sa.Integer(), nullable=False), + sa.ForeignKeyConstraint(['trpanel_id'], ['trpanels.id'], ), + sa.PrimaryKeyConstraint('id') + ) + op.create_index(op.f('ix_repeats_chr'), 'repeats', ['chr'], unique=False) + op.create_index(op.f('ix_repeats_divergence'), 'repeats', ['divergence'], unique=False) + op.create_index(op.f('ix_repeats_end'), 'repeats', ['end'], unique=False) + op.create_index(op.f('ix_repeats_id'), 'repeats', ['id'], unique=False) + op.create_index(op.f('ix_repeats_l_effective'), 'repeats', ['l_effective'], unique=False) + op.create_index(op.f('ix_repeats_motif'), 'repeats', ['motif'], unique=False) + op.create_index(op.f('ix_repeats_msa'), 'repeats', ['msa'], unique=False) + op.create_index(op.f('ix_repeats_n_effective'), 'repeats', ['n_effective'], unique=False) + op.create_index(op.f('ix_repeats_p_value'), 'repeats', ['p_value'], unique=False) + op.create_index(op.f('ix_repeats_region_length'), 'repeats', ['region_length'], unique=False) + op.create_index(op.f('ix_repeats_score'), 'repeats', ['score'], unique=False) + op.create_index(op.f('ix_repeats_score_type'), 'repeats', ['score_type'], unique=False) + op.create_index(op.f('ix_repeats_source'), 'repeats', ['source'], unique=False) + op.create_index(op.f('ix_repeats_start'), 'repeats', ['start'], unique=False) + op.create_index(op.f('ix_repeats_trpanel_id'), 'repeats', ['trpanel_id'], unique=False) + op.create_table('transcripts', + sa.Column('id', sa.Integer(), nullable=False), + sa.Column('ensembl_transcript', sqlmodel.sql.sqltypes.AutoString(), nullable=False), + sa.Column('start', sa.Integer(), nullable=False), + sa.Column('end', sa.Integer(), nullable=False), + sa.Column('gene_id', sa.Integer(), nullable=True), + sa.ForeignKeyConstraint(['gene_id'], ['genes.id'], ), + sa.PrimaryKeyConstraint('id'), + sa.UniqueConstraint('ensembl_transcript') + ) + op.create_index(op.f('ix_transcripts_end'), 'transcripts', ['end'], unique=False) + op.create_index(op.f('ix_transcripts_ensembl_transcript'), 'transcripts', ['ensembl_transcript'], unique=False) + op.create_index(op.f('ix_transcripts_gene_id'), 'transcripts', ['gene_id'], unique=False) + op.create_index(op.f('ix_transcripts_id'), 'transcripts', ['id'], unique=False) + op.create_index(op.f('ix_transcripts_start'), 'transcripts', ['start'], unique=False) + op.create_table('allele_frequencies', + sa.Column('id', sa.Integer(), nullable=False), + sa.Column('population', sqlmodel.sql.sqltypes.AutoString(), nullable=False), + sa.Column('n_effective', sa.Integer(), nullable=False), + sa.Column('frequency', sa.Float(), nullable=False), + sa.Column('het', sa.Float(), nullable=True), + sa.Column('num_called', sa.Integer(), nullable=True), + sa.Column('repeat_id', sa.Integer(), nullable=False), + sa.ForeignKeyConstraint(['repeat_id'], ['repeats.id'], ), + sa.PrimaryKeyConstraint('id') + ) + op.create_index(op.f('ix_allele_frequencies_frequency'), 'allele_frequencies', ['frequency'], unique=False) + op.create_index(op.f('ix_allele_frequencies_het'), 'allele_frequencies', ['het'], unique=False) + op.create_index(op.f('ix_allele_frequencies_id'), 'allele_frequencies', ['id'], unique=False) + op.create_index(op.f('ix_allele_frequencies_n_effective'), 'allele_frequencies', ['n_effective'], unique=False) + op.create_index(op.f('ix_allele_frequencies_num_called'), 'allele_frequencies', ['num_called'], unique=False) + op.create_index(op.f('ix_allele_frequencies_population'), 'allele_frequencies', ['population'], unique=False) + op.create_index(op.f('ix_allele_frequencies_repeat_id'), 'allele_frequencies', ['repeat_id'], unique=False) + op.create_table('crcvariations', + sa.Column('id', sa.Integer(), nullable=False), + sa.Column('instable_calls', sa.Integer(), nullable=True), + sa.Column('stable_calls', sa.Integer(), nullable=True), + sa.Column('total_calls', sa.Integer(), nullable=True), + sa.Column('frac_variable', sa.Float(), nullable=True), + sa.Column('avg_size_diff', sa.Float(), nullable=True), + sa.Column('repeat_id', sa.Integer(), nullable=False), + sa.ForeignKeyConstraint(['repeat_id'], ['repeats.id'], ), + sa.PrimaryKeyConstraint('id') + ) + op.create_index(op.f('ix_crcvariations_avg_size_diff'), 'crcvariations', ['avg_size_diff'], unique=False) + op.create_index(op.f('ix_crcvariations_frac_variable'), 'crcvariations', ['frac_variable'], unique=False) + op.create_index(op.f('ix_crcvariations_id'), 'crcvariations', ['id'], unique=False) + op.create_index(op.f('ix_crcvariations_instable_calls'), 'crcvariations', ['instable_calls'], unique=False) + op.create_index(op.f('ix_crcvariations_repeat_id'), 'crcvariations', ['repeat_id'], unique=False) + op.create_index(op.f('ix_crcvariations_stable_calls'), 'crcvariations', ['stable_calls'], unique=False) + op.create_index(op.f('ix_crcvariations_total_calls'), 'crcvariations', ['total_calls'], unique=False) + op.create_table('exons_transcripts', + sa.Column('exon_id', sa.Integer(), nullable=True), + sa.Column('transcript_id', sa.Integer(), nullable=True), + sa.ForeignKeyConstraint(['exon_id'], ['exons.id'], ), + sa.ForeignKeyConstraint(['transcript_id'], ['transcripts.id'], ), + sa.PrimaryKeyConstraint('exon_id', 'transcript_id') + ) + op.create_index(op.f('ix_exons_transcripts_exon_id'), 'exons_transcripts', ['exon_id'], unique=False) + op.create_index(op.f('ix_exons_transcripts_transcript_id'), 'exons_transcripts', ['transcript_id'], unique=False) + op.create_table('genes_repeats', + sa.Column('repeat_id', sa.Integer(), nullable=True), + sa.Column('gene_id', sa.Integer(), nullable=True), + sa.ForeignKeyConstraint(['gene_id'], ['genes.id'], ), + sa.ForeignKeyConstraint(['repeat_id'], ['repeats.id'], ), + sa.PrimaryKeyConstraint('repeat_id', 'gene_id') + ) + op.create_index(op.f('ix_genes_repeats_gene_id'), 'genes_repeats', ['gene_id'], unique=False) + op.create_index(op.f('ix_genes_repeats_repeat_id'), 'genes_repeats', ['repeat_id'], unique=False) + op.create_table('repeats_transcripts', + sa.Column('repeat_id', sa.Integer(), nullable=True), + sa.Column('transcript_id', sa.Integer(), nullable=True), + sa.ForeignKeyConstraint(['repeat_id'], ['repeats.id'], ), + sa.ForeignKeyConstraint(['transcript_id'], ['transcripts.id'], ), + sa.PrimaryKeyConstraint('repeat_id', 'transcript_id') + ) + op.create_index(op.f('ix_repeats_transcripts_repeat_id'), 'repeats_transcripts', ['repeat_id'], unique=False) + op.create_index(op.f('ix_repeats_transcripts_transcript_id'), 'repeats_transcripts', ['transcript_id'], unique=False) + # ### end Alembic commands ### + + +def downgrade(): + # ### commands auto generated by Alembic - please adjust! ### + op.drop_index(op.f('ix_repeats_transcripts_transcript_id'), table_name='repeats_transcripts') + op.drop_index(op.f('ix_repeats_transcripts_repeat_id'), table_name='repeats_transcripts') + op.drop_table('repeats_transcripts') + op.drop_index(op.f('ix_genes_repeats_repeat_id'), table_name='genes_repeats') + op.drop_index(op.f('ix_genes_repeats_gene_id'), table_name='genes_repeats') + op.drop_table('genes_repeats') + op.drop_index(op.f('ix_exons_transcripts_transcript_id'), table_name='exons_transcripts') + op.drop_index(op.f('ix_exons_transcripts_exon_id'), table_name='exons_transcripts') + op.drop_table('exons_transcripts') + op.drop_index(op.f('ix_crcvariations_total_calls'), table_name='crcvariations') + op.drop_index(op.f('ix_crcvariations_stable_calls'), table_name='crcvariations') + op.drop_index(op.f('ix_crcvariations_repeat_id'), table_name='crcvariations') + op.drop_index(op.f('ix_crcvariations_instable_calls'), table_name='crcvariations') + op.drop_index(op.f('ix_crcvariations_id'), table_name='crcvariations') + op.drop_index(op.f('ix_crcvariations_frac_variable'), table_name='crcvariations') + op.drop_index(op.f('ix_crcvariations_avg_size_diff'), table_name='crcvariations') + op.drop_table('crcvariations') + op.drop_index(op.f('ix_allele_frequencies_repeat_id'), table_name='allele_frequencies') + op.drop_index(op.f('ix_allele_frequencies_population'), table_name='allele_frequencies') + op.drop_index(op.f('ix_allele_frequencies_num_called'), table_name='allele_frequencies') + op.drop_index(op.f('ix_allele_frequencies_n_effective'), table_name='allele_frequencies') + op.drop_index(op.f('ix_allele_frequencies_id'), table_name='allele_frequencies') + op.drop_index(op.f('ix_allele_frequencies_het'), table_name='allele_frequencies') + op.drop_index(op.f('ix_allele_frequencies_frequency'), table_name='allele_frequencies') + op.drop_table('allele_frequencies') + op.drop_index(op.f('ix_transcripts_start'), table_name='transcripts') + op.drop_index(op.f('ix_transcripts_id'), table_name='transcripts') + op.drop_index(op.f('ix_transcripts_gene_id'), table_name='transcripts') + op.drop_index(op.f('ix_transcripts_ensembl_transcript'), table_name='transcripts') + op.drop_index(op.f('ix_transcripts_end'), table_name='transcripts') + op.drop_table('transcripts') + op.drop_index(op.f('ix_repeats_trpanel_id'), table_name='repeats') + op.drop_index(op.f('ix_repeats_start'), table_name='repeats') + op.drop_index(op.f('ix_repeats_source'), table_name='repeats') + op.drop_index(op.f('ix_repeats_score_type'), table_name='repeats') + op.drop_index(op.f('ix_repeats_score'), table_name='repeats') + op.drop_index(op.f('ix_repeats_region_length'), table_name='repeats') + op.drop_index(op.f('ix_repeats_p_value'), table_name='repeats') + op.drop_index(op.f('ix_repeats_n_effective'), table_name='repeats') + op.drop_index(op.f('ix_repeats_msa'), table_name='repeats') + op.drop_index(op.f('ix_repeats_motif'), table_name='repeats') + op.drop_index(op.f('ix_repeats_l_effective'), table_name='repeats') + op.drop_index(op.f('ix_repeats_id'), table_name='repeats') + op.drop_index(op.f('ix_repeats_end'), table_name='repeats') + op.drop_index(op.f('ix_repeats_divergence'), table_name='repeats') + op.drop_index(op.f('ix_repeats_chr'), table_name='repeats') + op.drop_table('repeats') + op.drop_index(op.f('ix_cohorts_trpanel_id'), table_name='cohorts') + op.drop_index(op.f('ix_cohorts_name'), table_name='cohorts') + op.drop_index(op.f('ix_cohorts_id'), table_name='cohorts') + op.drop_table('cohorts') + op.drop_index(op.f('ix_trpanels_name'), table_name='trpanels') + op.drop_index(op.f('ix_trpanels_method'), table_name='trpanels') + op.drop_index(op.f('ix_trpanels_id'), table_name='trpanels') + op.drop_index(op.f('ix_trpanels_genome_id'), table_name='trpanels') + op.drop_table('trpanels') + op.drop_index(op.f('ix_genes_strand'), table_name='genes') + op.drop_index(op.f('ix_genes_start'), table_name='genes') + op.drop_index(op.f('ix_genes_name'), table_name='genes') + op.drop_index(op.f('ix_genes_id'), table_name='genes') + op.drop_index(op.f('ix_genes_genome_id'), table_name='genes') + op.drop_index(op.f('ix_genes_entrez_id'), table_name='genes') + op.drop_index(op.f('ix_genes_ensembl_version_id'), table_name='genes') + op.drop_index(op.f('ix_genes_ensembl_id'), table_name='genes') + op.drop_index(op.f('ix_genes_end'), table_name='genes') + op.drop_index(op.f('ix_genes_description'), table_name='genes') + op.drop_index(op.f('ix_genes_chr'), table_name='genes') + op.drop_table('genes') + op.drop_index(op.f('ix_genomes_version'), table_name='genomes') + op.drop_index(op.f('ix_genomes_organism'), table_name='genomes') + op.drop_index(op.f('ix_genomes_name'), table_name='genomes') + op.drop_index(op.f('ix_genomes_id'), table_name='genomes') + op.drop_table('genomes') + op.drop_index(op.f('ix_exons_stop_codon'), table_name='exons') + op.drop_index(op.f('ix_exons_start_codon'), table_name='exons') + op.drop_index(op.f('ix_exons_start'), table_name='exons') + op.drop_index(op.f('ix_exons_id'), table_name='exons') + op.drop_index(op.f('ix_exons_ensembl_exon'), table_name='exons') + op.drop_index(op.f('ix_exons_end'), table_name='exons') + op.drop_index(op.f('ix_exons_cds'), table_name='exons') + op.drop_table('exons') + # ### end Alembic commands ### \ No newline at end of file diff --git a/database_setup/migrations/versions/2023_08_27_1759-edc492faa8d0_.py b/database_setup/migrations/versions/2023_08_27_1759-edc492faa8d0_.py new file mode 100644 index 0000000..87550ad --- /dev/null +++ b/database_setup/migrations/versions/2023_08_27_1759-edc492faa8d0_.py @@ -0,0 +1,31 @@ +"""empty message + +Revision ID: edc492faa8d0 +Revises: 31a133f5e181 +Create Date: 2023-08-27 17:59:36.552503 + +""" + +# revision identifiers, used by Alembic. +revision = 'edc492faa8d0' +down_revision = '31a133f5e181' + +from alembic import op +import sqlalchemy as sa +import sqlmodel + + +def upgrade(): + # ### commands auto generated by Alembic - please adjust! ### + with op.batch_alter_table('genomes', schema=None) as batch_op: + batch_op.create_unique_constraint('ix_genome_name_unique_constraint', ['name']) + + # ### end Alembic commands ### + + +def downgrade(): + # ### commands auto generated by Alembic - please adjust! ### + with op.batch_alter_table('genomes', schema=None) as batch_op: + batch_op.drop_constraint('ix_genome_name_unique_constraint', type_='unique') + + # ### end Alembic commands ### \ No newline at end of file diff --git a/database_setup/migrations/versions/2023_09_03_1728-09734b51018c_.py b/database_setup/migrations/versions/2023_09_03_1728-09734b51018c_.py new file mode 100644 index 0000000..d79d55b --- /dev/null +++ b/database_setup/migrations/versions/2023_09_03_1728-09734b51018c_.py @@ -0,0 +1,53 @@ +"""empty message + +Revision ID: 09734b51018c +Revises: edc492faa8d0 +Create Date: 2023-09-03 17:28:21.869967 + +""" + +# revision identifiers, used by Alembic. +revision = '09734b51018c' +down_revision = 'edc492faa8d0' + +from alembic import op +import sqlalchemy as sa +import sqlmodel + + +def upgrade(): + # ### commands auto generated by Alembic - please adjust! ### + op.create_table('crc_expr_repeatlen_corr', + sa.Column('repeat_id', sa.Integer(), nullable=True), + sa.Column('gene_id', sa.Integer(), nullable=True), + sa.Column('p_value', sa.Float(), nullable=False), + sa.Column('p_value_corrected', sa.Float(), nullable=False), + sa.Column('coefficient', sa.Float(), nullable=False), + sa.Column('intercept', sa.Float(), nullable=False), + sa.ForeignKeyConstraint(['gene_id'], ['genes.id'], ), + sa.ForeignKeyConstraint(['repeat_id'], ['repeats.id'], ), + sa.PrimaryKeyConstraint('gene_id', 'repeat_id'), + ) + with op.batch_alter_table('crc_expr_repeatlen_corr', schema=None) as batch_op: + batch_op.create_index(batch_op.f('ix_crc_expr_repeatlen_corr_coefficient'), ['coefficient'], unique=False) + batch_op.create_index(batch_op.f('ix_crc_expr_repeatlen_corr_gene_id'), ['gene_id'], unique=False) + batch_op.create_index(batch_op.f('ix_crc_expr_repeatlen_corr_intercept'), ['intercept'], unique=False) + batch_op.create_index(batch_op.f('ix_crc_expr_repeatlen_corr_p_value'), ['p_value'], unique=False) + batch_op.create_index(batch_op.f('ix_crc_expr_repeatlen_corr_p_value_corrected'), ['p_value_corrected'], unique=False) + batch_op.create_index(batch_op.f('ix_crc_expr_repeatlen_corr_repeat_id'), ['repeat_id'], unique=False) + + # ### end Alembic commands ### + + +def downgrade(): + # ### commands auto generated by Alembic - please adjust! ### + with op.batch_alter_table('crc_expr_repeatlen_corr', schema=None) as batch_op: + batch_op.drop_index(batch_op.f('ix_crc_expr_repeatlen_corr_repeat_id')) + batch_op.drop_index(batch_op.f('ix_crc_expr_repeatlen_corr_p_value_corrected')) + batch_op.drop_index(batch_op.f('ix_crc_expr_repeatlen_corr_p_value')) + batch_op.drop_index(batch_op.f('ix_crc_expr_repeatlen_corr_intercept')) + batch_op.drop_index(batch_op.f('ix_crc_expr_repeatlen_corr_gene_id')) + batch_op.drop_index(batch_op.f('ix_crc_expr_repeatlen_corr_coefficient')) + + op.drop_table('crc_expr_repeatlen_corr') + # ### end Alembic commands ### \ No newline at end of file diff --git a/docker-compose.yml b/docker-compose.yml new file mode 100644 index 0000000..d68c433 --- /dev/null +++ b/docker-compose.yml @@ -0,0 +1,55 @@ +version: '3.4' + +services: + db: + image: postgres:13 + environment: + - PGDATA=/var/lib/postgresql/data/pgdata + - PGUSER=webstr + - POSTGRES_USER=webstr + - POSTGRES_PASSWORD=webstr + - POSTGRES_DB=webstr + volumes: + - './db/docker_data:/var/lib/postgresql/data' + ports: + - 5432:5432 + healthcheck: + test: ["CMD", "pg_isready", "-q"] + interval: 10s + timeout: 5s + retries: 5 + + api: + env_file: + - .env + image: webstr_api + build: + context: . + dockerfile: ./Dockerfile + ports: + - 5000:5000 + depends_on: + db: + condition: service_healthy + volumes: + - '.:${WEBSTR_SOURCE_MOUNT_PATH:-/tmp/src}' # not mount of the sourcecode if not set in .env + healthcheck: + test: ["CMD", "curl", "-f", "http://localhost:5000"] + interval: 1m30s + timeout: 10s + retries: 3 + start_period: 40s + + frontend: + environment: + - WEBSTR_API_URL=http://api:5000 + - FLASK_PORT=5001 + build: https://github.com/gymrek-lab/webstr.git#main + depends_on: + api: + condition: service_healthy + ports: + - 5001:5001 + + + diff --git a/entrypoint.sh b/entrypoint.sh new file mode 100644 index 0000000..06d8207 --- /dev/null +++ b/entrypoint.sh @@ -0,0 +1,26 @@ +#!/bin/bash + +if [ ${WEBSTR_DATABASE_MIGRATE:-0} == "True" ] +then + echo "Running database upgrade ${DATABASE_URL}" + python -m alembic upgrade head +else + echo "Skipping database upgrade" +fi + +if [ ${WEBSTR_DATABASE_DATA_UPGRADE:-0} == "True" ] +then + echo "Running database data insert ${DATABASE_URL}" + cd database_setup + bash full_db_setup.sh + cd .. +else + echo "Skipping database data insert" +fi + +if [ ${WEBSTR_DEVELOPMENT:-0} == "True" ] +then + uvicorn strAPI.main:app --host=0.0.0.0 --port=${PORT:-5000} --log-level debug --reload --reload-include strAPI +else + uvicorn strAPI.main:app --host=0.0.0.0 --port=${PORT:-5000} +fi \ No newline at end of file diff --git a/requirements.txt b/requirements.txt index 090e334..ddc9229 100644 --- a/requirements.txt +++ b/requirements.txt @@ -7,23 +7,18 @@ ipaddr==2.2.0 keyring==23.2.1 mock==4.0.3 mypy_extensions==0.4.3 -numpy==1.21.2 ordereddict==1.1 protobuf==3.18.0 pydantic==1.8.2 pyOpenSSL==20.0.1 simplejson==3.17.5 SQLAlchemy==1.4.23 -#starlette>0.14 starlette==0.14.2 unicodedata2==13.0.0.post2 -wincertstore==0.2 -uvicorn>0.9 -alembic>1.7.1 psycopg2-binary>2.9 sqlmodel==0.0.4 aiofiles==22.1.0 -alembic==1.8.1 +alembic==1.11.3 Brotli==1.0.9 cffi==1.15.1 chardet==4.0.0 @@ -55,7 +50,9 @@ sqlalchemy2-stubs==0.0.2a29 sqlmodel==0.0.4 starlette==0.14.2 typing_extensions==4.4.0 -unicodedata2==13.0.0.post2 uvicorn==0.20.0 wincertstore==0.2 zipp==3.11.0 +mygene==3.2.2 +pandas==2.0.3 +gtfparse==2.0.1 \ No newline at end of file diff --git a/strAPI/main.py b/strAPI/main.py index feed7e8..4559a00 100644 --- a/strAPI/main.py +++ b/strAPI/main.py @@ -1,6 +1,16 @@ import os import io import csv +import logging +import sys +from sqlalchemy.orm import joinedload + +# workaround to make relative imports work with __main__ +if __name__ == '__main__': + from os.path import dirname + __path__ = [dirname(__file__)] + del dirname + from . import genes as gn from typing import List, Optional @@ -12,11 +22,12 @@ from starlette.responses import RedirectResponse from fastapi.responses import StreamingResponse +import sqlalchemy from sqlmodel import Session, select from sqlalchemy import nullslast from .repeats import models, schemas -from .repeats.database import get_db, engine +from .repeats.database import get_db # this is not needed if using alembic #models.Base.metadata.create_all(bind=engine) @@ -432,3 +443,18 @@ def show_transcripts(gene: str, db: Session = Depends(get_db)): def get_sorted_exons(transcript: str, protein: bool = False, db: Session = Depends(get_db)): return genes.get_exons_by_transcript(db, protein, transcript) +""" Retrieve all CRC Gene Expression Repeat Length Correlations + + Returns + List of correlations between genes and a specific repeat length in CRC patients +""" +@app.get("/crc_expr_repeatlen_corr/", response_model=List[schemas.CRCExprRepeatLenCorr]) +def get_crc_expr_repeatlen_corr(db: Session = Depends(get_db), limit = 7000): + + correlations = db.query(models.CRCExprRepeatLenCorr).order_by( + sqlalchemy.func.abs(models.CRCExprRepeatLenCorr.coefficient).desc() + ).limit(limit).options( + joinedload(models.CRCExprRepeatLenCorr.gene), joinedload(models.CRCExprRepeatLenCorr.repeat) + ).all() + + return [{**c.gene.__dict__, **c.repeat.__dict__, **c.__dict__} for c in correlations] \ No newline at end of file diff --git a/strAPI/repeats/database.py b/strAPI/repeats/database.py index 3e59167..0f43f8f 100644 --- a/strAPI/repeats/database.py +++ b/strAPI/repeats/database.py @@ -1,13 +1,62 @@ import os -from sqlmodel import create_engine, Session +import logging +import sys +from io import StringIO + +from sqlmodel import create_engine, Session, SQLModel + +from alembic.config import Config as AlembicConfig +from alembic.script import ScriptDirectory as AlembicScriptDirectory +from alembic.migration import MigrationContext DATABASE_URL = os.environ['DATABASE_URL'] # Convert "postgres://" --> "postgresql+psycopg2://" needed for SQLAlchemy -final_db_url = "postgresql+psycopg2://" + DATABASE_URL.lstrip("postgres://") +final_db_url = DATABASE_URL.replace("postgres://", "postgresql+psycopg2://") + +""" +WARNING: Alembic functionality was teste but not used by default. Treat it as a POC for future versions. + +Check the database version and check if migrations need to be executed +There is support for executing migratiosn right away but calling alembic tool is more reliable +""" +def check_db_version(engine, config_file: str = os.path.join(os.path.split(__file__)[0], "../../alembic.ini")) -> None: + + alembic_cfg = AlembicConfig(config_file) + script_location = os.path.join(os.path.split(config_file)[0], + alembic_cfg.get_section(alembic_cfg.config_ini_section)["script_location"] + ) + alembic_cfg.set_main_option('script_location', script_location) + script_directory = AlembicScriptDirectory.from_config(alembic_cfg) + + with engine.begin() as connection: + alembic_context = MigrationContext.configure(connection) + current_db_version = alembic_context.get_current_revision() + + expected_db_version = script_directory.get_current_head() + + db_version_msg: str = f"Database: version check. current: {current_db_version}, expected: {expected_db_version}" + logging.info(db_version_msg) + + if current_db_version == expected_db_version: + logging.info(f"Database: version match {expected_db_version}. {final_db_url}") + else: + logging.info(f"Database: version mismatch") + raise Exception(f""" + {db_version_msg} + You can use 'python -m alembic upgrade head' run migrations on the database. + If that fails though you will need to use alembic tool and alembic.ini file to perform addtitional actions + This can require changing database url in alembic.ini and running alembic upgrade head + """) engine = create_engine(final_db_url, echo=False) +""" +WARNING: Alembic functionality was teste but not used by default. Treat it as a POC for future versions. +""" +if os.environ.get("WEBSTR_DATABASE_MIGRATIONS_ENABLE", "") == "1": + check_db_version(engine) + def get_db(): with Session(engine) as session: yield session diff --git a/strAPI/repeats/models.py b/strAPI/repeats/models.py index 9bd7461..e5a235a 100644 --- a/strAPI/repeats/models.py +++ b/strAPI/repeats/models.py @@ -1,7 +1,6 @@ from typing import Optional, List, Dict -from sqlalchemy import Integer, CheckConstraint, UniqueConstraint +from sqlalchemy import Integer, CheckConstraint, UniqueConstraint, ForeignKeyConstraint from sqlmodel import SQLModel, Field, Relationship, JSON, Column - class ExonTranscriptsLink(SQLModel, table=True): __tablename__ = "exons_transcripts" @@ -31,7 +30,6 @@ class GenesRepeatsLink(SQLModel, table=True): ) - class Transcript(SQLModel, table=True): __tablename__ = "transcripts" __table_args__ = (UniqueConstraint("ensembl_transcript"),) @@ -51,6 +49,9 @@ class Transcript(SQLModel, table=True): # many to many Repeats <-> Transcripts repeats: List["Repeat"] = Relationship(back_populates="transcripts", link_model = RepeatTranscriptsLink) + class Config: + arbitrary_types_allowed = True + def __repr__(self): return "Transcript(ensembl_transcript={}, start={}, end={})".format( self.ensembl_transcript, @@ -83,6 +84,10 @@ class Gene(SQLModel, table=True): # many to many Gene <-> Repeat repeats: List["Repeat"] = Relationship(back_populates="genes", link_model = GenesRepeatsLink) + crc_expr_repeatlen_corr: Optional[List["CRCExprRepeatLenCorr"]] = Relationship( + back_populates="gene" + ) + def __repr__(self): return "Gene(ensembl_id={}, chr={}, strand={}, start={}, end={}, name={}, description={}, entrez_id={})".format( self.ensembl_id, @@ -133,6 +138,7 @@ class CRCVariation(SQLModel, table=True): # One to one, Repeat - CRCVariation repeat_id: int = Field(foreign_key = "repeats.id") repeat: "Repeat" = Relationship(back_populates="crcvariation") + """ Allele Frequencies for 1000 Genomes project populations @@ -206,6 +212,11 @@ class Repeat(SQLModel, table=True): sa_relationship_kwargs={'uselist': False}, back_populates="repeat" ) + + crc_expr_repeatlen_corr: Optional[List["CRCExprRepeatLenCorr"]] = Relationship( + back_populates="repeat" + ) + # Add relationship directive to Repeat class for one to many Repeat - AlleleFrequency allfreqs: Optional[List["AlleleFrequency"]] = Relationship( back_populates="repeat" @@ -295,6 +306,7 @@ def __repr__(self): """ class Genome(SQLModel, table=True): __tablename__ = "genomes" + __table_args__ = (UniqueConstraint("name", name = "ix_genome_name_unique_constraint"),) id: int = Field(default=None, primary_key=True) name: str = Field(nullable=False) @@ -312,9 +324,34 @@ def __repr__(self): ) +""" +Correlation of gene expression and length of STRs in cancer patients +""" +class CRCExprRepeatLenCorr(SQLModel, table=True): + __tablename__ = "crc_expr_repeatlen_corr" + + + repeat_id: Optional[int] = Field( + default=None, foreign_key="repeats.id", primary_key=True + ) + repeat: "Repeat" = Relationship(back_populates="crc_expr_repeatlen_corr") + gene_id: Optional[int] = Field( + default=None, foreign_key="genes.id", primary_key=True + ) + gene: "Gene" = Relationship(back_populates="crc_expr_repeatlen_corr") + p_value: float = Field(nullable=False) + p_value_corrected: float = Field(nullable=False) + coefficient: float = Field(nullable=False) + intercept: float = Field(nullable=False) + def __repr__(self): + return "CRCExprRepeatLenCorr(gene={}, repeat={}, p_value={})".format( + self.gene.ensembl_id, + self.repeat.start, + self.p_value + ) diff --git a/strAPI/repeats/schemas.py b/strAPI/repeats/schemas.py index c659d83..a64c8f7 100644 --- a/strAPI/repeats/schemas.py +++ b/strAPI/repeats/schemas.py @@ -2,7 +2,6 @@ from typing import Optional, List, Tuple from pydantic import BaseModel - class Gene(BaseModel): ensembl_id: str chr: str @@ -104,4 +103,24 @@ class Transcript(BaseModel): class Config: orm_mode = True +class CRCExprRepeatLenCorr(BaseModel): + + repeat_id: int + gene_id: int + coefficient: float + intercept: float + p_value: float + p_value_corrected: float + + ensembl_id: str + chr: str + start: int + + name: Optional[str] + description: Optional[str] + + class Config: + orm_mode = True + + \ No newline at end of file