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QCM test - Inlet port 3 - Outlet port 2 - 1 step - 90 cycles - 3 mins-cycle - Qdraw 300 pulses-sec Qpump 300 pulses-sec - Period 1 cycle.txt
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QCM test - Inlet port 3 - Outlet port 2 - 1 step - 90 cycles - 3 mins-cycle - Qdraw 300 pulses-sec Qpump 300 pulses-sec - Period 1 cycle.txt
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#-------------------------------------------------------
# QCM vapor pumping script
#
# Version: 0.4
# Author: Dimitrios Simatos
# Last updated: 2022-11-16
# Changes: Updated the duration of script with the measured duration of script.
#-------------------------------------------------------
# Parameters
#
# Input port sequence: 3
# Outlet port sequence: 2
# Number of steps (step: an integer number of cycles during which a specific liquid is injected): 1
# Number of cycles: 90
# Cycles per step: 90
# Cycle time: 3 minutes
# Period (number of cycles between pumping cycles - even of the same liquid): 1
# Liquid injections per step (cycles per step/period): 90
# Duration of script: Theoretical: 4.5 hours, Measured:
#
# Syringe size: 1 ml
# Step resolution: N = 0.01 mm
# Drawing flow (Qdraw): V = 300 pulses/second (6000 ul/min)
# Pumping flow (Qpump): V = 300 pulses/second (6000 ul/min)
#-------------------------------------------------------
# Description
#
# This script is designed to pump a liquid from a single port into the QCM.
# If sensing proteins, perform two additional steps:
# - Saturate the tubes for 1-2 hours with a highly concentrated protein solution.
# - Measure consecutive transfer curves for 4 hours, to allow the device to charge and the baseline to stabilize to its maximum value.
#-------------------------------------------------------
# Important points
#
# Never leave any liquid in the pump to dry after the experiments. If they dry, the residue may block the valve.
# Always run the cleaning script after the experiments.
# Use clockwise rotation when going up in valve port numbers and vice versa.
# Do not tighten the reservoir caps. It may lead to overpressure of air.
#-------------------------------------------------------
# Things to do before running the script
#
# If sensing proteins, saturate the tubes with a highly concentrated protein solution.
# Set time units to minutes.
# Set filenames.
# Replace the tubing, if necessary.
#-------------------------------------------------------
# Port connections
#
# Port 1: Waste
# Port 2: Device
# Port 3: DI water
# Port 4: Nothing
# Port 5: Nothing
# Port 6: Nothing
#-------------------------------------------------------
# Pumping cycles and execution time
#
# There is only 1 step, with 90 cycles.
# There are 90 pumping cycles, each one followed by 3 minutes of measurements.
# All cycles pump DI water from port 3.
#
# Execution time for a single cycle: 6 minutes
# Total (theoretical) execution time: 1 (initialization) + 1 x 90 x 3 = 271 minutes = 4.5 hours
# Total (measured) execution time:
#-------------------------------------------------------
# Volume of liquid needed
#
# Volume of liquid needed per cycle: 1 ml
# Total volume of liquid needed per script run (volume/step * steps in which the liquid is injected):
# 1 ml x 90 = 90 ml DI water
#-------------------------------------------------------
# Pump initialization
pump /1T #hard stop pump
pump /1ZR #initialize pump
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 1: Pumping the liquid of port 3
filename step_1_DI-H2O-vapor_initial_
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
wait-min 1 #wait 1 minute
pump /1N0R #set step resolution: 0.01 mm
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be at 0)
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
wait 1000 #wait 1 second
pump /1I3R #go to port 3, clockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1A3000R #go to absolute position 3000 (= draw 1 ml)
wait-min 1 #wait 1 minute
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should have finished moving)
#pump /1?6 #report valve position
pump /1O2R #switch to port 2, counterclockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
pump /1A0R #go to absolute position 0 (= empty syringe)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be moving to 0)
#pump /1?6 #report valve position
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 2: Pumping the liquid of port 3
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
wait-min 1 #wait 1 minute
pump /1N0R #set step resolution: 0.01 mm
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be at 0)
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
wait 1000 #wait 1 second
pump /1I3R #go to port 3, clockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1A3000R #go to absolute position 3000 (= draw 1 ml)
wait-min 1 #wait 1 minute
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should have finished moving)
#pump /1?6 #report valve position
pump /1O2R #switch to port 2, counterclockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
pump /1A0R #go to absolute position 0 (= empty syringe)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be moving to 0)
#pump /1?6 #report valve position
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 3: Pumping the liquid of port 3
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
wait-min 1 #wait 1 minute
pump /1N0R #set step resolution: 0.01 mm
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be at 0)
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
wait 1000 #wait 1 second
pump /1I3R #go to port 3, clockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1A3000R #go to absolute position 3000 (= draw 1 ml)
wait-min 1 #wait 1 minute
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should have finished moving)
#pump /1?6 #report valve position
pump /1O2R #switch to port 2, counterclockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
pump /1A0R #go to absolute position 0 (= empty syringe)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be moving to 0)
#pump /1?6 #report valve position
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 4: Pumping the liquid of port 3
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
wait-min 1 #wait 1 minute
pump /1N0R #set step resolution: 0.01 mm
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be at 0)
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
wait 1000 #wait 1 second
pump /1I3R #go to port 3, clockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1A3000R #go to absolute position 3000 (= draw 1 ml)
wait-min 1 #wait 1 minute
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should have finished moving)
#pump /1?6 #report valve position
pump /1O2R #switch to port 2, counterclockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
pump /1A0R #go to absolute position 0 (= empty syringe)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be moving to 0)
#pump /1?6 #report valve position
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 5: Pumping the liquid of port 3
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
wait-min 1 #wait 1 minute
pump /1N0R #set step resolution: 0.01 mm
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be at 0)
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
wait 1000 #wait 1 second
pump /1I3R #go to port 3, clockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1A3000R #go to absolute position 3000 (= draw 1 ml)
wait-min 1 #wait 1 minute
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should have finished moving)
#pump /1?6 #report valve position
pump /1O2R #switch to port 2, counterclockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
pump /1A0R #go to absolute position 0 (= empty syringe)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be moving to 0)
#pump /1?6 #report valve position
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 6: Pumping the liquid of port 3
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
wait-min 1 #wait 1 minute
pump /1N0R #set step resolution: 0.01 mm
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be at 0)
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
wait 1000 #wait 1 second
pump /1I3R #go to port 3, clockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1A3000R #go to absolute position 3000 (= draw 1 ml)
wait-min 1 #wait 1 minute
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should have finished moving)
#pump /1?6 #report valve position
pump /1O2R #switch to port 2, counterclockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
pump /1A0R #go to absolute position 0 (= empty syringe)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be moving to 0)
#pump /1?6 #report valve position
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 7: Pumping the liquid of port 3
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
wait-min 1 #wait 1 minute
pump /1N0R #set step resolution: 0.01 mm
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be at 0)
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
wait 1000 #wait 1 second
pump /1I3R #go to port 3, clockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1A3000R #go to absolute position 3000 (= draw 1 ml)
wait-min 1 #wait 1 minute
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should have finished moving)
#pump /1?6 #report valve position
pump /1O2R #switch to port 2, counterclockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
pump /1A0R #go to absolute position 0 (= empty syringe)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be moving to 0)
#pump /1?6 #report valve position
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 8: Pumping the liquid of port 3
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
wait-min 1 #wait 1 minute
pump /1N0R #set step resolution: 0.01 mm
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be at 0)
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
wait 1000 #wait 1 second
pump /1I3R #go to port 3, clockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1A3000R #go to absolute position 3000 (= draw 1 ml)
wait-min 1 #wait 1 minute
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should have finished moving)
#pump /1?6 #report valve position
pump /1O2R #switch to port 2, counterclockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
pump /1A0R #go to absolute position 0 (= empty syringe)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be moving to 0)
#pump /1?6 #report valve position
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 9: Pumping the liquid of port 3
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
wait-min 1 #wait 1 minute
pump /1N0R #set step resolution: 0.01 mm
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be at 0)
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
wait 1000 #wait 1 second
pump /1I3R #go to port 3, clockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1A3000R #go to absolute position 3000 (= draw 1 ml)
wait-min 1 #wait 1 minute
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should have finished moving)
#pump /1?6 #report valve position
pump /1O2R #switch to port 2, counterclockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
pump /1A0R #go to absolute position 0 (= empty syringe)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be moving to 0)
#pump /1?6 #report valve position
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 10: Pumping the liquid of port 3
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
wait-min 1 #wait 1 minute
pump /1N0R #set step resolution: 0.01 mm
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be at 0)
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
wait 1000 #wait 1 second
pump /1I3R #go to port 3, clockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1A3000R #go to absolute position 3000 (= draw 1 ml)
wait-min 1 #wait 1 minute
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should have finished moving)
#pump /1?6 #report valve position
pump /1O2R #switch to port 2, counterclockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
pump /1A0R #go to absolute position 0 (= empty syringe)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be moving to 0)
#pump /1?6 #report valve position
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 11: Pumping the liquid of port 3
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
wait-min 1 #wait 1 minute
pump /1N0R #set step resolution: 0.01 mm
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be at 0)
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
wait 1000 #wait 1 second
pump /1I3R #go to port 3, clockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1A3000R #go to absolute position 3000 (= draw 1 ml)
wait-min 1 #wait 1 minute
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should have finished moving)
#pump /1?6 #report valve position
pump /1O2R #switch to port 2, counterclockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
pump /1A0R #go to absolute position 0 (= empty syringe)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be moving to 0)
#pump /1?6 #report valve position
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 12: Pumping the liquid of port 3
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
wait-min 1 #wait 1 minute
pump /1N0R #set step resolution: 0.01 mm
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be at 0)
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
wait 1000 #wait 1 second
pump /1I3R #go to port 3, clockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1A3000R #go to absolute position 3000 (= draw 1 ml)
wait-min 1 #wait 1 minute
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should have finished moving)
#pump /1?6 #report valve position
pump /1O2R #switch to port 2, counterclockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
pump /1A0R #go to absolute position 0 (= empty syringe)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be moving to 0)
#pump /1?6 #report valve position
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 13: Pumping the liquid of port 3
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
wait-min 1 #wait 1 minute
pump /1N0R #set step resolution: 0.01 mm
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be at 0)
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
wait 1000 #wait 1 second
pump /1I3R #go to port 3, clockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1A3000R #go to absolute position 3000 (= draw 1 ml)
wait-min 1 #wait 1 minute
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should have finished moving)
#pump /1?6 #report valve position
pump /1O2R #switch to port 2, counterclockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
pump /1A0R #go to absolute position 0 (= empty syringe)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be moving to 0)
#pump /1?6 #report valve position
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 14: Pumping the liquid of port 3
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
wait-min 1 #wait 1 minute
pump /1N0R #set step resolution: 0.01 mm
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be at 0)
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
wait 1000 #wait 1 second
pump /1I3R #go to port 3, clockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1A3000R #go to absolute position 3000 (= draw 1 ml)
wait-min 1 #wait 1 minute
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should have finished moving)
#pump /1?6 #report valve position
pump /1O2R #switch to port 2, counterclockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
pump /1A0R #go to absolute position 0 (= empty syringe)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be moving to 0)
#pump /1?6 #report valve position
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 15: Pumping the liquid of port 3
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
wait-min 1 #wait 1 minute
pump /1N0R #set step resolution: 0.01 mm
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be at 0)
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
wait 1000 #wait 1 second
pump /1I3R #go to port 3, clockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1A3000R #go to absolute position 3000 (= draw 1 ml)
wait-min 1 #wait 1 minute
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should have finished moving)
#pump /1?6 #report valve position
pump /1O2R #switch to port 2, counterclockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
pump /1A0R #go to absolute position 0 (= empty syringe)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be moving to 0)
#pump /1?6 #report valve position
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 16: Pumping the liquid of port 3
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
wait-min 1 #wait 1 minute
pump /1N0R #set step resolution: 0.01 mm
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be at 0)
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
wait 1000 #wait 1 second
pump /1I3R #go to port 3, clockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1A3000R #go to absolute position 3000 (= draw 1 ml)
wait-min 1 #wait 1 minute
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should have finished moving)
#pump /1?6 #report valve position
pump /1O2R #switch to port 2, counterclockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
pump /1A0R #go to absolute position 0 (= empty syringe)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be moving to 0)
#pump /1?6 #report valve position
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 17: Pumping the liquid of port 3
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
wait-min 1 #wait 1 minute
pump /1N0R #set step resolution: 0.01 mm
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be at 0)
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
wait 1000 #wait 1 second
pump /1I3R #go to port 3, clockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1A3000R #go to absolute position 3000 (= draw 1 ml)
wait-min 1 #wait 1 minute
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should have finished moving)
#pump /1?6 #report valve position
pump /1O2R #switch to port 2, counterclockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
pump /1A0R #go to absolute position 0 (= empty syringe)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be moving to 0)
#pump /1?6 #report valve position
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 18: Pumping the liquid of port 3
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
wait-min 1 #wait 1 minute
pump /1N0R #set step resolution: 0.01 mm
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be at 0)
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
wait 1000 #wait 1 second
pump /1I3R #go to port 3, clockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1A3000R #go to absolute position 3000 (= draw 1 ml)
wait-min 1 #wait 1 minute
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should have finished moving)
#pump /1?6 #report valve position
pump /1O2R #switch to port 2, counterclockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
pump /1A0R #go to absolute position 0 (= empty syringe)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be moving to 0)
#pump /1?6 #report valve position
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 19: Pumping the liquid of port 3
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
wait-min 1 #wait 1 minute
pump /1N0R #set step resolution: 0.01 mm
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be at 0)
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
wait 1000 #wait 1 second
pump /1I3R #go to port 3, clockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1A3000R #go to absolute position 3000 (= draw 1 ml)
wait-min 1 #wait 1 minute
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should have finished moving)
#pump /1?6 #report valve position
pump /1O2R #switch to port 2, counterclockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
pump /1A0R #go to absolute position 0 (= empty syringe)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be moving to 0)
#pump /1?6 #report valve position
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 20: Pumping the liquid of port 3
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
wait-min 1 #wait 1 minute
pump /1N0R #set step resolution: 0.01 mm
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be at 0)
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
wait 1000 #wait 1 second
pump /1I3R #go to port 3, clockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1A3000R #go to absolute position 3000 (= draw 1 ml)
wait-min 1 #wait 1 minute
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should have finished moving)
#pump /1?6 #report valve position
pump /1O2R #switch to port 2, counterclockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
pump /1A0R #go to absolute position 0 (= empty syringe)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be moving to 0)
#pump /1?6 #report valve position
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 21: Pumping the liquid of port 3
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
wait-min 1 #wait 1 minute
pump /1N0R #set step resolution: 0.01 mm
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be at 0)
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
wait 1000 #wait 1 second
pump /1I3R #go to port 3, clockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1A3000R #go to absolute position 3000 (= draw 1 ml)
wait-min 1 #wait 1 minute
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should have finished moving)
#pump /1?6 #report valve position
pump /1O2R #switch to port 2, counterclockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
pump /1A0R #go to absolute position 0 (= empty syringe)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be moving to 0)
#pump /1?6 #report valve position
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 22: Pumping the liquid of port 3
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
wait-min 1 #wait 1 minute
pump /1N0R #set step resolution: 0.01 mm
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be at 0)
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
wait 1000 #wait 1 second
pump /1I3R #go to port 3, clockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1A3000R #go to absolute position 3000 (= draw 1 ml)
wait-min 1 #wait 1 minute
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should have finished moving)
#pump /1?6 #report valve position
pump /1O2R #switch to port 2, counterclockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
pump /1A0R #go to absolute position 0 (= empty syringe)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be moving to 0)
#pump /1?6 #report valve position
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 23: Pumping the liquid of port 3
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
wait-min 1 #wait 1 minute
pump /1N0R #set step resolution: 0.01 mm
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be at 0)
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
wait 1000 #wait 1 second
pump /1I3R #go to port 3, clockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1A3000R #go to absolute position 3000 (= draw 1 ml)
wait-min 1 #wait 1 minute
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should have finished moving)
#pump /1?6 #report valve position
pump /1O2R #switch to port 2, counterclockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
pump /1A0R #go to absolute position 0 (= empty syringe)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be moving to 0)
#pump /1?6 #report valve position
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 24: Pumping the liquid of port 3
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
wait-min 1 #wait 1 minute
pump /1N0R #set step resolution: 0.01 mm
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be at 0)
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
wait 1000 #wait 1 second
pump /1I3R #go to port 3, clockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1A3000R #go to absolute position 3000 (= draw 1 ml)
wait-min 1 #wait 1 minute
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should have finished moving)
#pump /1?6 #report valve position
pump /1O2R #switch to port 2, counterclockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
pump /1A0R #go to absolute position 0 (= empty syringe)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be moving to 0)
#pump /1?6 #report valve position
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 25: Pumping the liquid of port 3
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
wait-min 1 #wait 1 minute
pump /1N0R #set step resolution: 0.01 mm
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be at 0)
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
wait 1000 #wait 1 second
pump /1I3R #go to port 3, clockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1A3000R #go to absolute position 3000 (= draw 1 ml)
wait-min 1 #wait 1 minute
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should have finished moving)
#pump /1?6 #report valve position
pump /1O2R #switch to port 2, counterclockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
pump /1A0R #go to absolute position 0 (= empty syringe)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be moving to 0)
#pump /1?6 #report valve position
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 26: Pumping the liquid of port 3
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
wait-min 1 #wait 1 minute
pump /1N0R #set step resolution: 0.01 mm
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be at 0)
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
wait 1000 #wait 1 second
pump /1I3R #go to port 3, clockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1A3000R #go to absolute position 3000 (= draw 1 ml)
wait-min 1 #wait 1 minute
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should have finished moving)
#pump /1?6 #report valve position
pump /1O2R #switch to port 2, counterclockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
pump /1A0R #go to absolute position 0 (= empty syringe)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be moving to 0)
#pump /1?6 #report valve position
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 27: Pumping the liquid of port 3
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
wait-min 1 #wait 1 minute
pump /1N0R #set step resolution: 0.01 mm
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be at 0)
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
wait 1000 #wait 1 second
pump /1I3R #go to port 3, clockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1A3000R #go to absolute position 3000 (= draw 1 ml)
wait-min 1 #wait 1 minute
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should have finished moving)
#pump /1?6 #report valve position
pump /1O2R #switch to port 2, counterclockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
pump /1A0R #go to absolute position 0 (= empty syringe)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be moving to 0)
#pump /1?6 #report valve position
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 28: Pumping the liquid of port 3
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
wait-min 1 #wait 1 minute
pump /1N0R #set step resolution: 0.01 mm
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be at 0)
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
wait 1000 #wait 1 second
pump /1I3R #go to port 3, clockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1A3000R #go to absolute position 3000 (= draw 1 ml)
wait-min 1 #wait 1 minute
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should have finished moving)
#pump /1?6 #report valve position
pump /1O2R #switch to port 2, counterclockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
pump /1A0R #go to absolute position 0 (= empty syringe)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be moving to 0)
#pump /1?6 #report valve position
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 29: Pumping the liquid of port 3
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
wait-min 1 #wait 1 minute
pump /1N0R #set step resolution: 0.01 mm
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be at 0)
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
wait 1000 #wait 1 second
pump /1I3R #go to port 3, clockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1A3000R #go to absolute position 3000 (= draw 1 ml)
wait-min 1 #wait 1 minute
pump /1V300R #choose speed: 300 steps/s (6000 ul/min)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should have finished moving)
#pump /1?6 #report valve position
pump /1O2R #switch to port 2, counterclockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
pump /1A0R #go to absolute position 0 (= empty syringe)