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Add tag with pre-alignment score to unaligned reads #58

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jdidion opened this issue Sep 19, 2023 · 2 comments
Open

Add tag with pre-alignment score to unaligned reads #58

jdidion opened this issue Sep 19, 2023 · 2 comments

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@jdidion
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jdidion commented Sep 19, 2023

I set --pre-align-min-score 200 and had a read fail to align, however the read aligned with minimap2. When I dropped the threshold to 100, the read aligned.

It would be useful to know the pre-alignment scores of reads that are expected to align but fail to do so, so that --pre-align-min-score can be adjusted intelligently.

I don't see any reason why AS couldn't be used.

@nh13
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nh13 commented Sep 19, 2023

How about the XS or xs tag, with the latter as per the README:

the sub-optimal alignment score, practically the maximum of any pre-alignment and secondary chain

then one could get the xs tag for all reads

@jdidion
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jdidion commented Sep 19, 2023

perfect

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