fasterq-dump into six files?

[jeongyeonkim95]

Hi

I am trying to download and split files for SRR16784068.

In original file section, it says that this SRR file contains 6 fastq files (3 from RNA sequencing, 3 from BCR sequencing).

However, I can't seem to find a way to accurately divide these files into six parts.

Could you assist how I can do this using sra toolkit?

Thanks

[stineaj]

@jeongyeonkim95 It would be difficult to use the toolkit to split back into the 6 originally submitted fastq data. The data model for SRA would not generally expect that sequence data from two different sources like RNA and BCR would be merged into a single run. I think your best option currently would be to use CDDS to request the source files. We will also alert the curation staff at SRA to this run and they may be able to work with the submitter to determine if the data would be better presented if it was processed differently.